Always the right dose and color of light

ZEISS Viluma Family

Flexible and Bright Light Sources for Your Fluorescence Imaging

zeiss.com/viluma

Flexible and Bright Light Sources for Your Fluorescence Imaging

› In Brief Excited by the right wavelength, fluorescent labels give your biological samples the contrast you need for meaningful imaging. Light emitting diodes (LED) are

› The Advantages ideal for gentle live cell imaging: They only emit the part of the light spectrum

› The Applications that is required for the precise activation of your labels and avoid unwanted, ­

› The System cell-damaging UV leakage.

› Technology and Details ZEISS Viluma is a family of light sources for fluorescence imaging that provides

› Service you with all the benefits of advanced LED technology: Expect stable illumination to obtain reproducible data. Achieve results faster with high imaging speeds and millisecond switching times. Fine-tune the excitation dose for even your most sensitive samples by precisely attenuating each individual LED. With up to nine illumination channels, image more labels than ever before across the entire spectrum – from UV to far red. And in combination with a multi-bandpass filter, you can use your Viluma for automated multichannel imaging on any manual microscope. What’s more, as the LED light sources are switched off instantly whenever acquisition is paused, you not just save lamp time but also help to protect our environment.

2 Simpler.

More Intelligent. More Integrated. Powerful and Bright Adaptable and Easy to Use Precise and Gentle

› In Brief LEDs convert electrical power into light more Your ZEISS Viluma light source can house LEDs LEDs can be attenuated easily and do not emit

› The Advantages efficiently than other light sources. With ZEISS with a broad variety of wavelengths and inten- unwanted heat or cell-damaging UV light. Your Viluma, full excitation intensity is available over sities. Choose the configuration that exactly ZEISS Viluma houses specially designed and

› The Applications the whole lifetime without warm-up times. matches your applications and budget. With up selected filters that are optimized for excitation

› The System Improve the efficiency and reproducibility of yourto nine illumination channels from UV to far red, of all common dyes and fluorescent proteins with imaging experiments, as control over excitation isyou are not limited in your choice of labels. All highest accuracy. Your sample is only exposed to

› Technology and Details precise and very stable. You always get enoughLEDs were specifically selected to deliver exactly the radiation that is needed to excite the fluores-

› Service excitation power and can speed up your image the right wavelength and intensity for all your cent label. Use ZEN imaging software and hard- acquisition, if necessary.experiments in life sciences research. You control ware triggers to control excitation intensity andZEISS Viluma with the familiar ZEN imaging soft-wavelength with microsecond precision, perfectlyware, the ­control panel, or the TFT of your ZEISS synchronized with your image acquisition. Yourmicroscope. sensitive sample is only exposed to light when aframe is acquired.100 90 80 BP 340/30Absolute Transmission [%] 70 BP 387/15 60 BP 423/44 50 BP 469/38 40 BP 508/20 30 BP 555/30 20 BP 591/27 10 BP 631/33 0 BP 735/40 300 400 500 600 700 800 Wavelength [nm]Human tonsil tissue stained with multiple cancer markers

3 The Viluma Family

Depending on your experiments, the requirements for a light source vary. Your science may require several excitation channels for imaging multiple colors. Or you

› In Brief want to excite fluorophores with highest intensity to shorten exposure times for improving throughput or frame rate. The ZEISS Viluma family was designed with the

› The Advantages various requirements of your applications in mind.

› The Applications All ZEISS Viluma light sources are characterized by stable performance immediately after switching on. They are optimally adapted to ZEISS microscopes and provide

› The System highest homogeneity across the field of view. State-of-the-art LEDs with high light output guarantee always enough power for imaging dim samples or with high frame rates. Thanks to the integration into the ZEN imaging suite, even complex experiments can be conducted in an automated way.

› Technology and Details

› Service ZEISS Viluma 5ZEISS Viluma 7ZEISS Viluma 9 Your affordable LED light source for fluorescence Your flexible and fast LED light source for gentleYour versatile LED light source for most microscopylive cell imaging ­demanding applications from UV to far red• 4 excitation channels• Up to 7 excitation channels• Up to 9 excitation channels• Directly coupled to stand• Directly coupled to stand• Coupled to stand via liquid light guide• Total light output: 940 mW• Total light output: 1510 mW• Total light output: ~5600 mW

4 The Viluma Family

The light sources of the ZEISS Viluma family can Viluma 5 Viluma 7Viluma 9

› In Brief be used on various ZEISS microscopes to address Replacement of mercury vapor light sources or other classical light sources

› The Advantages a multitude of applications. Short switching Multi-color imaging with multi and single band filter sets times and bright LEDs are beneficial for routine Fast and gentle multi color imaging

› The Applications inspections of staining procedures, whole mount 2D and 3D imaging with ZEISS Apotome

› The System imaging, efficient large-area tissue screening, orQuantitative Imaging in cell physiology or immunohistochemistry gentle live cell imaging. Select your ZEISS Viluma 3D widefield imaging and deconvolution

› Technology and Details light source depending on your requirements and Long-term time lapse imaging

› Service applications. Routine fluorescence imaging visual observation FRET imaging or other dual excitation imaging experiments Demanding multi-color FISH applications Whole mount imaging Fura-2 calcium imaging High-speed multi-color imaging of large-area tissue and multiwell plates Traditional white light sources produce a Viluma 7 vs HXP 120 continuous spectrum with high intensity peaks at 1.0 some wavelengths and lower intensity for others. 0.9 They require warm-up time and regular bulb 0.8 UV changes to ensure a stable light output. State-of- 0.7 V the-art LED technology produces light matchingNormalized Intensity 0.6B or exceeding classical white light sources for 0.5C most of the visible spectrum. Additionally, they 0.4G allow to extend the excitation spectrum to far red 0.3 Y R which was previously inaccessible. Mercury-free 0.2 FR components with long-lasting performance avoid 0.1 HXP toxic consumables. Full performance is available 0 350 400 450 500 550 600 650 700 750 800 directly after start at significantly lower running Wavelength / nm costs.

5 Your Insight into the Technology Behind It

ZEISS Viluma 9

› In Brief Your versatile LED light source for most demanding applications from UV to far red

› The Advantages• Excitation spectrum from UV to far red: Up to 9 illumination ­channels

› The Applications cover all important dyes, fluorescent proteins and near UV probes like Fura-2

› The System• Laser-driven phosphor: A laser-excited phosphor ensures highest light­output in the range from 500 nm to 600 nm.

› Technology and Details• Integrated filter wheel: Up to 4 filters can be used to define distinct bands

› Service in the spectrum of the phosphor emission.• Quiet operation: LED cooling is realized by efficient thermal design and­low-noise fans to ensure comfortable imaging.• Flexible placement of the light source: Coupling via liquid light guide(LLG) allows to position the light source anywhere close to the microscope.• Simultaneous use of all LEDs without restriction: According to therequirements of your application, you can switch on as many LEDs as needed(or even all of them) at once.• speedDIAL: Illumination channel and intensity can be controlled directly on thedevice. A dedicated display provides information about the status of the LED.

6 Your Insight into the Technology Behind It

ZEISS Viluma 9 allows you to excite your labels

› In Brief with up to 9 different wavelengths. Two models100

› The Advantages optimized for excitation of near UV or far-red 90 dyes cover the entire visible spectrum. A laser- 80 BP 340/30

› The Applications Absolute Transmission [%] powered phosphor creates bright excitation in the 70 BP 387/15

› The System range between 500 nm and 600 nm where LEDs 60 BP 423/44 typically lack intensity. ZEISS Viluma 9 comes with

› Technology and Details 50 BP 469/38 a light guide giving you the flexibility to use it also with sensitive electrophysiologic setups or with 40 BP 508/20

› Service zoom microscopes like ZEISS Axio Zoom.V16. 30 BP 555/30 20 BP 591/27 Available excitation lines of Viluma 9 are exactly 10 BP 631/33 centered around the common dyes and fluores- 0 BP 735/40 cent proteins. 300 400 500 600700 800 Wavelength [nm]Built-in excitation filters of ZEISS Viluma 9 models Available excitation bands for ZEISS Viluma 9 Line Wavelength / BandwidthRecommended Dye (Examples) UV 1 340 / 30 nm Fura-2, Alexa 350, (Amino) Coumari, Indo-1, Marina Blue, DAPI, Hoechst, LysoSensor Yellow, SBFI-Na+, Dansyl Cadaverine, AMCA conjugate UV2385 / 30 nm DAPI, Hoechst 33342, Hoechst 33258, Alexa Fluor 350, Alexa Fluor 405, Indo-1, eBFP / BFP, eGFP (wt), True Blue UV 1 387 / 15 nm DAPI, Hoechst 33342, Hoechst 33258, Alexa Fluor 350, Alexa Fluor 405, Indo-1, eBFP / BFP, eGFP (wt), True Blue V 423 / 44 nm Pacific Blue, Lucifer Yellow, Alexa Fluor 433, eCFP, Cerulean B 469 / 38 nm FM1-43, Cy2, eGFP, NBD, MitoTracker Green, Alexa Fluor 488, Fluo-4, BCECF, Calcein, DiO SNAFL, YO-Pro-1, Nissl, LysoSensor Green, mHoneydew, FITC / Fluorescein, Kaede (green / red), PerCP, YoYo-1, FuraRed C 508 / 20 nm Rhodamine 123, Oregon Green BAPTA, Sytox Green, eYFP, FM4-64, Eosin / HE, Acridine Orange, JC1, Bodipy FL, Propidium Iodide, Spectrum Green, Calcium Green G 555 / 30 nm TRITC, 7-AAD, Cy3, tdTomato, Alexa Fluor 546, Alexa Fluor 555, DsRed, mOrange, TagRFP, SNARF, DyLight 549, Spectrum Orange Y 591 / 27 nm MitoTracker RED FM/CMXRos, txRed, mCherry, mRFP1, Cy3.5, Rhodamine B, Alexa Fluor 568, Dylight 594, Alexa Fluor 594, Bodipy TR R 631 / 33 nm Alexa Fluor 633, Alexa Fluor 647, Cy5, DRAQ5, ToTo-3, ATTO-655, MitoTracker DeepRed, APC, ATTO-647N FR 2 735 / 40 nm Alexa Fluor 750, Alexa Fluor 790, Cy7, Cy7.5 1 Viluma 9 UV 2Viluma 9 VIS7 Your Insight into the Technology Behind It ZEISS Viluma 7

› In Brief Your flexible and fast LED light source for gentle live cell imaging

› The Advantages• Very broad-spectrum excitation: Up to 7 illumination channels cover• Long-term power stabilization and calibration: While booting,

› The Applications all important dyes, fluorescent proteins and probes.maximum LED power is measured and compared to a factory value. The

› The System• Matched filters: To avoid crosstalk and increase excitation efficiency of current of each LED is then adjusted to the internal reference to ensure aselected dyes, the excitation filters precisely match the LED emission spectra. constant light output over the entire lifetime of the light source.

› Technology and Details For increased flexibility, single and multi-bandpass filters are available.• Real-time control: As long as only one LED is turned on, the photodiode

› Service• Efficient light transfer: Directly coupled to the microscope and designed acts as a closed-loop power stabilization in the µs range. The power of thewith reduced optical elements, Viluma 7 ensures optimal light transfer. active LED is controlled even during the exposure time of an image. This• Compact housing, low noise: Viluma 7 consumes as little space asguarantees a stable light output even if the LED is used in short µs ranging­possible and will not disturb your work environment with noise or vibrations. exposures.• Closed-loop temperature control: A vibration-decoupled fan is com-bined with a special on-chip cooling design. The fan is controlled via directfeedback from the LEDs.• Precise excitation control: Directly control Viluma 7 through controlpanel and microscope TFT. Use the camera trigger for control to getfastest image acquisition. Precise hardware synchronization through TTLtriggering and perfect integration into ZEN imaging experiments let you useall the excitation light that reaches the sample for image acquisition.• Linearized LEDs: Any value you adjust the LED to corresponds exactly tothe power that the LED emits. A 50 % reduction will result in precisely halfthe power than running the LED at 100 %.• On-chip temperature sensing: Each LED emitter is equipped with anon-board temperature sensor to precisely measure the current temperaturein the millisecond range. 8 Your Insight into the Technology Behind It With ZEISS Viluma 7, you use up to six differ-

› In Brief ent LEDs to generate up to seven fluorescence 100

› The Advantages excitation lines for your sample. All LEDs besides 90 the Green / Yellow line [G / Y] come with individual 80

› The Applications excitation filters. The [G / Y] line uses a lime LEDBP 385/30Absolute Transmission [%] 70

› The System (567 / 100 nm) and a motorized filter changer toBP 423/44 emit green (555 nm) or yellow (591 nm) light on60

› Technology and Details BP 469/38 demand.50 BP 508/20

› Service 40 Available excitation lines of ZEISS Viluma 7 areBP 555/30 30 exactly centered around the common dyes and BP 591/27 20 fluorescent proteins. BP 631/33 10 BP 735/40 0 350 450 550650 750 Wavelength [nm]Built-in excitation filters of ZEISS Viluma 7 Available excitation bands for ZEISS Viluma 7 LineWavelength / Bandwidth Recommended Dye (Examples) UV 385 / 30 nm DAPI, Hoechst 33342, Hoechst 33258, Alexa Fluor 350, Alexa Fluor 405, Indo-1, eBFP / BFP, eGFP (wt), True Blue V 423 / 44 nm Pacific Blue, Lucifer Yellow, Alexa Fluor 433, eCFP, Cerulean B 469 / 38 nm FM1-43, Cy2, eGFP, NBD, MitoTracker Green, Alexa Fluor 488, Fluo-4, BCECF, Calcein, DiO SNAFL, YO-Pro-1, Nissl, LysoSensor Green, mHoneydew, FITC / Fluorescein, Kaede (green / red), PerCP, YoYo-1,FuraRed C 508 / 20 nm Rhodamine 123, Oregon Green BAPTA, Sytox Green, eYFP, FM4-64, Eosin / HE, Acridine Orange, JC1, Bodipy FL, Propidium Iodide, Spectrum Green, Calcium Green G 555 / 30 nm TRITC, 7-AAD, Cy3, tdTomato, Alexa Fluor 546, Alexa Fluor 555, DsRed, mOrange, TagRFP, SNARF, DyLight 549, Spectrum Orange Y 591 / 27 nm MitoTracker RED FM/CMXRos, txRed, mCherry, mRFP1, Cy3.5, Rhodamine B, Alexa Fluor 568, Dylight 594, Alexa Fluor 594, Bodipy TR R 631 / 33 nm Alexa Fluor 633, Alexa Fluor 647, Cy5, DRAQ5, ToTo-3, ATTO-655, MitoTracker DeepRed, APC, ATTO-647N FR 735 / 40 nm Alexa Fluor 750, Alexa Fluor 790, Cy7, Cy7.5 9 Your Insight into the Technology Behind It ZEISS Viluma 5

› In Brief Your affordable LED light source for fluorescence microscopy

› The Advantages• Broad-spectrum excitation: Four illumination channels cover the most

› The Applications important dyes, fluorescent proteins, and probes.

› The System• Matched filters: To avoid crosstalk and increase excitation efficiency ofselected dyes, the excitation filters precisely match the LED emission spectra.

› Technology and Details For increased flexibility, single and multi-bandpass filters are available.

› Service• Efficient light transfer: Directly coupled to the microscope and designedwith reduced optical elements, Viluma 5 ensures optimal light transfer.• Compact housing, low noise: Viluma 5 consumes as little space as­possible and will not disturb your work environment with noise or vibrations.• Closed-loop temperature control: A vibration-decoupled fan is­combined with a special on-chip cooling design. The fan is controlled viadirect feedback from the LEDs.• Precise excitation control: Directly control Viluma 5 through controlpanel and microscope TFT. Use the camera trigger for control to getfastest image acquisition. Precise hardware synchronization through TTL­triggering and perfect integration into ZEN imaging experiments let you useall the excitation light that reaches the sample for image acquisition.• Linearized LEDs: Any value you adjust the LED to corresponds exactly tothe power that the LED emits. A 50 % reduction will result in precisely halfthe power than running the LED at 100 %.• On-chip temperature sensing: Each LED emitter is equipped with anon-board temperature sensor to precisely measure the current temperaturein the millisecond range. 10 Your Insight into the Technology Behind It With Viluma 5, you can use four different LEDs

› In Brief 100 for fluorescence excitation of your sample. All90

› The Advantages LEDs come with individual excitation filters. The available excitation lines of Viluma 5 are exactly80 Absolute Transmission [%]

› The Applications centered around the most common dyes and 70

› The System fluorescent proteins. 60 50

› Technology and Details 40

› Service 30 20 10 0 350 400 450 500 550 600650 Wavelength [nm] BP 385/30 BP 469/38 BP 555/30 BP 631/33 Built-in excitation filters of ZEISS Viluma 5 Available excitation bands for ZEISS Viluma 5 Line Wavelength / Bandwidth Recommended Dye (Examples) UV 385 / 30 nmDAPI, Hoechst 33342, Hoechst 33258, Alexa Fluor 350, Alexa Fluor 405, Indo-1, eBFP / BFP, eGFP (wt), True Blue B 469 / 38 nmFM1-43, Cy2, eGFP, NBD, MitoTracker Green, Alexa Fluor 488, Fluo-4, BCECF, Calcein, DiO SNAFL, YO-Pro-1, Nissl, LysoSensor Green, mHoneydew, FITC / Fluorescein,Kaede (green / red), PerCP, YoYo-1, FuraRed G 555 / 30 nmTRITC, 7-AAD, Cy3, tdTomato, Alexa Fluor 546, Alexa Fluor 555, DsRed, mOrange, TagRFP, SNARF, DyLight 549, Spectrum Orange R 631 / 33 nmAlexa Fluor 633, Alexa Fluor 647, Cy5, DRAQ5, ToTo-3, ATTO-655, MitoTracker DeepRed, APC, ATTO-647N 11 ZEISS Viluma Family at Work

› In Brief

› The Advantages

› The Applications

› The System

› Technology and Details

› Service 1000 μm Mouse embryo sagittal cut, embryonic day E13, 12 µm. SOX2 stained with Alexa488, Pax6 stainedwith Cy3, Nestin stained with Alexa647, Nuclei with DAPI. Sample courtesy: Ivan Mestres,TU Dresden, Germany 50 μm10 µm thick murine lung tissue section with tumor metastasis. Sample courtesy of H. Ishikawa-Anker- Mouse kidney in fluorescence, cryosection, AF 488 – WGA, AF 568 Phalloidin, DAPI, objective: Plan- hold, Walter-Brendel-Zentrum für Experimentelle Medizin München, Germany Apochromat 20× / 0.8 12 ZEISS Viluma Family at Work

› In Brief

› The Advantages

› The Applications

› The System

› Technology and Details

› Service Immunohistochemistry staining of a E16.5 mouse brain section. Nuclei were labled with DAPI, Pax6 with Alexa488, Tbr2 with Alexa 561 and Dach1 with Alexa647. Sample courtesy of Da Mi, Tsinghua University, China. HEK cells stained with DAPI, phalloidin-488, tubulinA568 and WGA-A633 (maximum intensity projec- HEK cells stained with DAPI, phalloidin-488, tubulinA568 and WGA-A633 (maximum tion after deconvolution). intensity projection after deconvolution).13 ZEISS Viluma Family at Work

› In Brief

› The Advantages

› The Applications

› The System

› Technology and Details

› Service Fixed cultured HeLa Kyoto cells stained with Hoechst 33342 (blue), Aurora B – Alexa 488 (green), Tubulin – Alexa 568 (red) and ACA – A ­ lexa Living cultured pig kidney cells (LLC-PK1) with Emerald-Tubulin 647 (white). Image stack was deconvolved using ZEN Deconvolution module. Sample courtesy of Dr. A. Girod, University of Luxembourg. fusion (green) and mCherry-H2B fusion (red). Sample courtesy ofM. Davidson, Florida State University. Fixed cultured HeLa Kyoto cells stained with Hoechst 33342 Fixed cultured HeLa Kyoto cells stained with Hoechst 33342 Fixed cultured HeLa Kyoto cells stained with Hoechst 33342 (blue), Importin β – Alexa 488 (green) and Tubulin – Alexa 647 (blue), Importin β – Alexa 488 (green) and Tubulin – Alexa 647 (blue), Aurora B – Alexa 488 (green), phospho-Histone H3 – (red). Image stack was deconvolved using ZEN Deconvolution (red). Image stack was deconvolved using ZEN Deconvolution ­Alexa 568 (orange), Tubulin – Alexa 647 (red). Image stack was module. Sample courtesy of Dr. A. Girod, University of Luxem- module. Sample courtesy of Dr. A. Girod, University of Luxem- deconvolved using ZEN Deconvolution module. Sample courtesy bourg. bourg. of Dr. A. Girod, University of Luxembourg.14 ZEISS Viluma Family at Work

› In Brief

› The Advantages

› The Applications

› The System

› Technology and Details

› Service 500 µm3 µm section of a formalin fixed paraffin-embedded human lung. Section was stained with a 14 CODEX multiplex antibody panel. Image shows some essential markers to distinguish mononuclear phagocytes (CD45 in gray, CD68 in red, HLA-DR in green, CD11c in magenta). To delineate major anatomical structures, such as bronchioles and blood vessels, Pancytokeratin (yellow) and CD31 (cyan) are shown. Blue signals correspond to DAPI nuclear staining. Acquisition was done with a ZEISS Axio Observer microscope and processing with the Akoya CODEX Processor. Image featured in ZEISS Blog Article: "The Intricate Network of Immune Cells Revealed by Multiplex Tissue Microscopy" Courtesy of D. Bejarano, Life and Medical Sciences Institute (LIMES) of the University of Bonn, Germany 15 ZEISS Viluma Family at Work

› In Brief

› The Advantages

› The Applications

› The System

› Technology and Details

› Service 20 μm Click here to view this video Fura-2-loaded cells of the adrenal cell line Hac15 were continuously perfused with physiological Calculate intensity ratios for quantitatively determining intracellular Calcium responses directly in extracellular solution and then stimulated firstly with a high-potassium solution and secondly with ZEN. The Physiology Toolkit allows evaluation of live-cell experiments without tedious data in- and an ATP-containing solution. Intracellular Ca2+-responses were analyzed as the emission ratio of the export. Ca2+-free (340 nm) and Ca2+-bound (380 nm) signals. 16 Your Flexible Choice of Components

› In Brief

› The Advantages

› The Applications

› The System

› Technology and Details

› Service 3 Software ZEN, recommended Toolkits• Motorized Acquisition, Advanced Acquisition• 2D, 3D, Connect• Molecular Quantification• Deconvolution• AI, Bio Apps 4 Control 1 Microscopes 2 Filters• ZEN• Axio Imager A selection of high efficiency filter sets were• Control Panel• Axio Observer ­specifically designed for Viluma light sources:• Microscope TFT• Axio Examiner• FS 56 HE LED• TTL Triggering• Axioscan• FS 90 HE LED• Axio Zoom.V16• FS 91 HE LED 5 Others• FS 92 HE LED For maximum sample throughput and spectral• FS 108 HE LEDflexibility in excitation and emission, Viluma light• FS 109 HE LEDsources can be combined with the Axio Observer• FS 110 HE LEDdual filter wheel (452358-9011-000) for indepen-• FS 112 HE LEDdent switching of dichroic and emission filters.17 System Overview Viluma 5 / 7

› In Brief

› The Advantages

› The Applications

› The System

› Technology and Details

› Service Solid-State Light Source Viluma 5,Type RGB-UVwith 4 solid state LED lamps423052-9641-000included in delivery:- illumination module,- desktop power supply and- country-specific power cableFilter set 90 HE LED489090-9110-000 Solid-State Light Source Viluma 7,Type RGB-UVwith 4 solid state LED lamps423052-9711-000Solid-State Light Source Viluma 7,Type RYB-UVwith 4 solid state LED lamps423052-9721-000Solid-State Light Source Viluma 7, included in delivery: LED Trigger Box for Viluma 5/7 Type R[G/Y]B-UV - illumination module, 423052-9760-000with 5 solid state LED lamps - desktop power supply and423052-9731-000 - country-specific power cable (for fast control of theSolid-State Light Source Viluma 7, illumination module via TTLType R[G/Y]CBV-UV and analog signals from externalwith 7 solid state LED lamps software packages is needed)Control Panel for Viluma 5/7423052-9742-000423052-9751-000Solid-State Light Source Viluma 7,(for manual stands without Type FR-R[G/Y]BV-UVTFT control panel or software control with 7 solid state LED lampsis needed) 423052-9771-000 18 System Overview Viluma 9

› In Brief

› The Advantages

› The Applications

› The System

› Technology and Details Light source Viluma 9 VIS (XT920Z) 423006-9020-000

› Service Light source Viluma 9 UV (XT930Z) 423006-9030-000 Illumination adapter Viluma 9 423006-9060-000 Control panel speedDIAL Viluma 9 423006-9040-000 19 Technical Specifications Viluma 5 / 7 LED light source for fluorescence applications

› In Brief Up to 7 individually controlled excitation lines (Viluma 7) Linearized intensity regulation in increments of 1 % (from 1 – 100 %)

› The Advantages Standby mode to reduce power consumption

› The Applications Real-time stabilization of brightness for low-noise image captures (Viluma 7) Long-time stabilization and performance optimization to improve comparability of image captures (Viluma 7)

› The System Integrated and motorized (Viluma 7) switchable excitation filters Control via the TFT display of Axio Observer, Axio Examiner and Axio Imager

› Technology and Details Optional control via ZEN Imaging Software, manual control panel or Trigger Box

› Service Easy integration in 3rd party software packages via LED Trigger Box Guaranteed lifetime of 15,000 h per LED line Integrated interface for triggered image capture from ZEN Imaging SoftwareDimensions Light source (length × width × height) 174 mm × 107 mm × 182 mm LED Trigger Box (length × width × height) 220 mm × 245 mm × 105 mm Control panel (length × width × height)155 mm × 195 mm × 60 mmWeight Light source up to 2,100 g LED Trigger Boxapprox. 2,100 g Control panel approx. 590 gLight source model Order number Wavelength / Bandwidth (nm) Light Source Viluma 5 RGB-UV 423052-9641-000385 / 30, 469 / 38, 555 / 30, 631 / 33 Light Source Viluma 7 RGB-UV 423052-9711-000385 / 30, 469 / 38, 555 / 30, 631 / 33 Light Source Viluma 7 RYB-UV 423052-9721-000385 / 30, 469 / 38, 591 / 27, 631 / 33 Light Source Viluma 7 R[G / Y]B-UV 423052-9731-000385 / 30, 469 / 38, 555 / 30, 591 / 27, 631 / 33 Light Source Viluma 7 R[G / Y]CBV-UV 423052-9742-000385 / 30, 423 / 44, 469 / 38, 508 / 20, 555 / 30, 591 / 27, 631 / 33 Light Source Viluma 7 FR-R[G / Y]BV-UV 423052-9771-000385 / 30, 423 / 44, 469 / 38, 555 / 30, 591 / 27, 631 / 33, 735 / 40AccessoriesOrder number Comment Control panel for Viluma 5 / 7 423052-9751-000optional, for manual control LED trigger box forViluma 5 / 7423052-9760-000optional, for triggering of Viluma 5 / 7 with third-party hardware20 Technical Specifications Viluma 5 / 7 Ambient conditions

› In Brief Transport (in packaging) Permissible ambient temperature –20 °C to +55 °C

› The Advantages

› The Applications Storage Permissible ambient temperature +10 °C to +40 °C

› The System Permissible relative air humidity (no condensation) < 75 %

› Technology and Details Operation

› Service Permissible ambient temperature +10 °C to +40 °C Permissible relative air humidity (no condensation) < 75 % Altitude of operating sitemax. 2000 m Atmospheric pressure 800 hPa to 1060 hPa Degree of pollution 2Operating data Operating areaEnclosed rooms Protection class I Electrical safety in compliance with DIN EN 61010-1 (IEC 61010-1) and conforming to CSA and UL regulations Overvoltage category II Noise immunityin accordance with DIN EN 61326-1 Line voltage of controller module 100 to 240 VAC (±10 %) Line frequency50 to 60 Hz Current consumption 1.9 A 21 Technical Specifications Viluma 9 LED light source for fluorescence applications

› In Brief Up to 9 individually controlled excitation lines Patented LaserLED Hybrid Drive for high-power excitation from 500 – 600 nm

› The Advantages Build-in excitation filters, switchable by integrated filter wheel

› The Applications Intensity regulation in increments of 0.5 % (from 1 – 100 %) Compatible with Axio Observer, Axio Imager, Axio Examiner and Axio Zoom.V16

› The System Control via ZEN Imaging Software, control panel speedDIAL or triggering Integrated interface for triggered image capture from ZEN Imaging Software

› Technology and Details Guaranteed lifetime of 25,000 hrs or 3 years for each LED and LaserLED Hybrid Drive

› Service Dimensions Light source (length × width × height)265 mm × 205 mm × 270 mm Control panel speedDIAL (length × width × height) 59 mm × 80 mm × 112 mmWeight Light source 9 kg Control panel speedDIAL 300 gLight source modelOrder number Wavelength / Bandwidth (nm) Light Source Viluma 9 VIS (XT920Z)423006-9020-000385 / 30, 423 / 44, 469 / 38, 500 – 600, 508 / 20, 555 / 30, 591 / 27, 631 / 33, 735 / 40 Light Source Viluma 9 UV (XT930Z) 423006-9030-000340 / 30, 387 / 15, 423 / 44, 469 / 38, 500 – 600, 508 / 20, 555 / 30, 591 / 27, 631 / 33Accessories Order number Comment Control panel speedDIAL Viluma 9 423006-9040-000optional, for manual control Trigger cable Viluma 9 w single LED ports 423006-9050-000optional, for triggering of Viluma 9 with 3rd party hardware Trigger cable Viluma 9423006-9070-000optional, for triggering with SVB-1 or synchronization with camera Illumination adapter Viluma 9 430006-9060-000for maximized intensity and homogeneity 22 Technical Specifications Viluma 9 Ambient conditions

› In Brief Transport (in packaging) Permissible ambient temperature –35 °C to +60 °C

› The Advantages

› The Applications Storage Permissible relative air humidity (no condensation) 10 % to 95 %

› The System Atmospheric pressure 500 hPa to 1060 hPa

› Technology and Details Operation

› Service Permissible ambient temperature +10 °C to +30 °C Altitude of operating sitemax. 2000 m Atmospheric pressure 700 to 1060 hPa Permissible relative air humidity (no condensation) 15 % to 90 % Installation category II Degree of pollution 2Operating data Operating areaEnclosed rooms Input voltage 100 – 240 VAC, 50 / 60 Hz Current 4.5 A max / 100 V, 1.9 A max / 240 V Input surge With hot or cold start, 20 A max ProtectionOver load and over temperature 23 Technical Specifications Filter Sets for ZEISS Viluma Light Sources

› In Brief Most filter sets are available with reflector module by exchanging 000 with 400 in the order number

› The Advantages Multi-bandpass filter sets for fast acquisition

› The Applications 489056-9110-000 489090-9110-000 489091-9110-000

› The System Filter set 56 HE LED (E)Filter set 90 HE LED (E) Filter set 91 HE LED (E) for use with ZEISS LED fluorescence light sources. for use with ZEISS LED fluorescence light sources.for use with ZEISS LED fluorescence light sources.

› Technology and Details Suitable for fluorescent dyes like GFP and DsRedSuitable for fluorescent dyes like DAPI, FITC, TRITC and Cy5 Suitable for fluorescent dyes like CFP, YFP and mCherry with excitation wavelengths 475 and 555 nm. with excitation wavelengths 385, 475, 555 and 630 nm. with excitation wavelengths 430, 508 and 590 nm.

› Service Contains beam splitter DBS 490 + 575 andContains beam splitter QBS 405 + 493 + 575 + 653 and Contains beam splitter TBS 450 + 538 + 610 and emission filter DBP 512 / 30 + 630 / 98.emission filter QBP 425 / 30 + 514 / 30 + 592 / 25 + 709 / 100. emission filter TBP 467 / 24 + 555 / 25 + 687 / 145. 489092-9110-000 489108-9110-000 489110-9111-000 Filter set 92 HE LED (E)Filter set 108 HE LED (E) Filter Set 110 HE LED (E) for use with ZEISS LED fluorescence light sources. for use with ZEISS LED fluorescence light sources.for use with ZEISS LED fluorescence light sources. Suitable for fluorescent dyes like DAPI, GFP and mCherrySuitable for fluorescent dyes like CFP and YFPSuitable for fluorescent dyes like DAPI, FITC, mCherry and Cy7 with excitation wavelengths 385, 475 and 590 nm.with excitation wavelengths 425 and 508 nm. with excitation wavelengths 385, 475, 590 and 735 nm. Contains beam splitter TBS 405 + 493 + 610 and Contains beam splitter DBS 450 + 538 and Contains beam splitter QBS 405 + 493 + 611 + 762 and emission filter TBP 425 / 30 + 524 / 50 + 688 / 145.emission filter DBP 467 / 24 + 598 / 110. emission filter QBP 425 / 30 + 524 / 51 + 634 / 38 + 785 / 38. 489112-9110-000 Filter Set 112 HE LED (E) for use with ZEISS LED fluorescence light sources. Suitable for fluorescent dyes like DAPI, FITC, DsRed, Cy5 and Cy7 with excitation wavelengths 385, 475, 555, 630 and 735 nm. Contains beam splitter PBS 405 + 493 + 575 + 654 + 761 and emission filter PBP 425 / 30 + 514 / 31 + 592 / 25 + 681 / 45 + 785 / 38. 24 Technical Specifications Filter Sets for ZEISS Viluma Light Sources

› In Brief Most filter sets are available with reflector module by exchanging 000 with 400 in the order number

› The Advantages Multi-bandpass filter sets with single emission filters for filter wheels

› The Applications 489056-9120-000 489090-9120-000 489091-9120-000

› The System Filter Set 56 LED SBP GFP+DsRed Filter Set 90 LED SBP DAPI+GFP+DsRed+Cy5 Filter Set 91 LED SBP CFP+YFP+AF594 for use with ZEISS LED fluorescence light sources and emission for use with ZEISS LED fluorescence light sources and emission for use with ZEISS LED fluorescence light sources and emission

› Technology and Details filter wheelfilter wheel filter wheel Consisting of: Consisting of: Consisting of:

› Service BS 490 + 575BS 405 + 493 + 575 + 653 BS 450 + 538 + 610 EM BP 514 / 30 EM BP 425 / 30 EM BP 467 / 24 EM BP 630 / 98 EM BP 514 / 30 EM BP 558 / 25 EM BP 592 / 25 EM BP 649 / 65 EM BP 681 / 45 489092-9120-000 489110-9120-000 489112-9120-000 Filter Set 92 LED SBP DAPI+GFP+AF594Filter Set 110 LED SBP DAPI+GFP+AF594+Cy7Filter Set 112 LED SBP DAPI+GFP+DsRed+Cy5+Cy7 for use with ZEISS LED fluorescence light sources and emission for use with ZEISS LED fluorescence light sources and emission for use with ZEISS LED fluorescence light sources and emission filter wheelfilter wheel filter wheel Consisting of: Consisting of: Consisting of: BS 405 + 493 + 610 BS 405 + 493 + 611 + 762 BS 405 + 493 + 575 + 654 + 761 EM BP 425 / 30 EM BP 425 / 30 EM BP 425 / 30 EM BP 525 / 50 EM BP 525 / 50 EM BP 514 / 30 EM BP 649 / 65 EM BP 649 / 65 EM BP 592 / 25 EM BP 788 / 38 EM BP 681 / 45 EM BP 788 / 38 Fura-2 Imaging 489021-0000-000 Filter set 21 HE Fura 2 shift free (E) EX BP 340 / 30, EX BP 387 / 15, BS FT 409, EM BP 510 / 90, recommended for white light fluorescence light sources or ZEISS LED fluorescence light sources. 25 Technical Specifications UV UV UV V B C G Y R FR

› In Brief Order No. Filter Set340 / 30 387 / 15 385 / 30 423 / 44 469 / 38 508 / 20 555 / 30 591 / 27 631 / 33 735 / 40 489021-0000-000 21 HE  

› The Advantages 489096-9100-000 96 HE  

› The Applications 489070-0000-000 70 HE 489038-9901-000 38 HE 

› The System 489118-9100-000 118

› Technology and Details 000000-1114-101 43 489043-9901-000 43 HE 

› Service 000000-1114-462 45 489121-9100-000 121  489064-0000-000 64 HE  488050-9901-000 50  489115-9100-000 115  489117-9140-000 117 FRET  489090-9110-000 90 HE LED      489090-9120-000 90 LED SBP      489091-9110-000 91 HE LED   489091-9120-000 91 LED SBP    489092-9110-000 92 HE LED    489092-9120-000 92 LED SBP    489056-9110-000 56 HE LED   489056-9120-000 56 LED SBP   489108-9110-000 108 HE LED   489109-9110-000 109 HE LED     489110-9111-000 110 HE LED     489110-9120-000 110 LED SBP    489112-9110-000 112 HE LED       489112-9120-000 112 LED SBP      26 Technical Specifications RGB-UVRYB-UV R[G / Y]B-UV R[G / Y]CBV-UV FR-R[G / Y]BV-UV Viluma 9 UV Viluma 9 VIS

› In Brief 423052-9641 423052-9721 423052-9731 423052-9742 423052-9771 423006-9030 423006-9020 Order No. Filter Set423052-9711

› The Advantages 489021-0000-000 21 HE  489096-9100-000 96 HE      

› The Applications 489070-0000-000 70 HE    

› The System 489038-9901-000 38 HE       489118-9100-000 118   

› Technology and Details 000000-1114-101 43   

› Service 489043-9901-000 43 HE     000000-1114-462 45    489121-9100-000 121     489064-0000-000 64 HE      488050-9901-000 50      489115-9100-000 115   489117-9140-000 117 FRET     489090-9110-000 90 HE LED     489090-9120-000 90 LED SBP    489091-9110-000 91 HE LED ()   489091-9120-000 91 LED SBP     489092-9110-000 92 HE LED      489092-9120-000 92 LED SBP      489056-9110-000 56 HE LED     489056-9120-000 56 LED SBP    489108-9110-000 108 HE LED ()   489109-9110-000 109 HE LED    489110-9111-000 110 HE LED   489110-9120-000 110 LED SBP    489112-9110-000 112 HE LED  489112-9120-000 112 LED SBP   27 Technical Specifications System Requirements ZEISS Viluma 5, 7 and 9

› In Brief All Vilumas can be run under ZEN 3.10 with MTB 3.9.4.0 or newer (triggering of Viluma 9 requires ZEN 3.11) LEDs and excitation filters are preconfigured and no customer interface

› The Advantages

› The Applications ZEISS Viluma 5 and ZEISS Viluma 7 Compatibility Notes Control via TFT is available for Axio Observer 3 / 5 / 7, Axio Examiner.Z and Axio Imager (M, Mm, Z andZm)

› The System To run the Viluma 5 / 7 from the TFT, the following firmware (for Main2 or Main3) is required: Axio Observer: 2.723 or 2.825

› Technology and Details Axio Imager: 4.587 or 4.888

› Service Axio Examiner: 3.545 or 3.841ZEISS Viluma 9 Compatibility Notes Control via TFT is not possible – use speedDIAL instead for manual control Requires a free USB port at the computer Switching between the excitation lines controlled with the internal filter wheel (500 – 600 nm) is slower than between all other lines Cer tified Q u a l i t y S y s te m ISO 13485 28 ZEISS Service – Your Partner at All Times Your microscope system from ZEISS is one of your most important tools. For over 175 years, the ZEISS brand and our experience have stood for reliable equipment with a long life in the field of microscopy. You can count on superior service and support - before and after installation. Our skilled ZEISS service team makes

› In Brief sure that your microscope is always ready for use.

› The Advantages

› The Applications ProcurementOperation• Lab Planning & Construction Site Management• Predictive Service Remote Monitoring

› The System• Site Inspection & Environmental Analysis• Inspection & Preventive Maintenance• GMP-Qualification IQ/OQ• Software Maintenance Agreements

› Technology and Details• Installation & Handover• Operation & Application Training

› Service• IT Integration Support• Expert Phone & Remote Support• Startup Training• Protect Service Agreements• Metrological Calibration• Instrument Relocation• Consumables• Repairs New Investment Retrofit• Decommissioning• Customized Engineering• Trade In• Upgrades & Modernization• Customized Workflows via ZEISS arivis Cloud Please note: Availability of services depends on product line and locationwww.zeiss.com/microscopy/service 29 07745 Jena, Germany [email protected] www.zeiss.com/viluma Carl Zeiss Microscopy GmbHFollow us on social media: Not all products are available in every country. Use of products for in vitro diagnostic procedures or purposes may be limited by local regulations. Contact your local ZEISS representative for more information. EN_41_011_330 | Version 1.0 | CZ 09-2024 | Design, scope of delivery, and technical progress subject to change without notice. | © Carl Zeiss Microscopy GmbH