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Explore advanced spectral unmixing techniques with the ZEISS LSM Plus. Advanced spectral multiplexing reveals detailed cell wall architecture in budding yeast, capturing 13 fluorescent labels plus autofluorescence in a single track

Unmixing Techniques with LSM: A Comprehensive Exploration

Key Takeaways

  • Research focus: Advanced spectral unmixing with ZEISS LSM Plus.
  • Model systems: Various biological samples for multichannel fluorescence imaging.
  • Research goal: Enhance clarity and quantitative analysis in complex datasets.
  • Presented by: ZEISS imaging experts.
  • Content type: Technical guide on imaging processing techniques.
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3 min read

Sample courtesy of Michal Skruzny, ZEISS Microscopy GmbH

Advanced spectral multiplexing of cell walls of budding yeast cells (Saccharomyces cerevisiae): 13 labels plus autofluorescence acquired in one track using 5 lasers and 36 detectors, unmixed image of the 13 labels without autofluorescence.

Sample courtesy of Michal Skruzny, ZEISS Microscopy GmbH


https://zeiss.widen.net/s/dltrlkjqfm/lambda_06_unmixing_lsm-plus-processing_final_allchannels
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