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The ZEISS Lightfield 4D provides cutting-edge imaging technologies, ideal for capturing real-time volumetric data. It features advancements crucial for dynamic imaging applications. Contact your local ZEISS representative for availability and more details.

Add ZEISS Lightfield 4D to your LSM 9 System

Key Takeaways

  • Research focus: Real-time volumetric imaging with Lightfield 4D.
  • Model system: Apt for dynamic biological studies.
  • Research goal: Capture and analyze complex temporal changes in samples.
  • Presented by: ZEISS informative guide.
  • Content type: Product Overview for advanced imaging technologies.
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2 min read

Lightfield 4D Upgrade for LSM 9 Series Systems


Enable your LSM with gentle instant volume imaging.


Employ light-field microscopy for instant volumetric imaging to study organism dynamics at up to 80 volumes per second, while preserving full spatiotemporal information. This approach enables the acquisition of thousands of volumes over time without harming living samples, and allows multiple positions of organisms, organoids, or spheroids to be captured in a single run. The unique one-snap-one-volume acquisition can also be seamlessly combined with any other imaging mode of your ZEISS LSM 9-series system.


zeiss.com/lightfield-4d


Benefits & Application


Investigating the morphology and cardiac wall


  1. High-speed physiological and neuronal processes movement of the developing zebrafish heart captured in 3D
  2. Gentle observation of entire organisms over extended periods of time
  3. Accelerated collection of information on large samples with multiple labels
  4. Innovative experimental design through high-speed volume imaging combined with all the possibilities of LSM


ZEN Microscopy Software


Unlock the full potential of ZEN's features, delivering improved precision, stability, better user experience, and new possibilities for imaging and data analysis.


Acquire images using smart automation

Process images with scientifically proven algorithms

Visualize big data by a GPU powered 3D engine

Analyze images via Machine Learning-based tools


mCherry expression in cardiomyocytes acquired with ZEISS LSM 990 Lightfield 4D and Plan-Apochromat 20× / 0.8 Air. Volume size: 723 × 723 × 430 µm³, exposure time 12 ms for 1.2 seconds in total. Sample courtesy of Stone Elworthy and Emily Noël, School of Biosciences, University of Sheffield, UK. Data acquired at Wolfson Light Microscopy Facility in the School of Biosciences at the University of Sheffield.


ZEISS arivis Pro


Achieve quantitative and reproducible results by automation of image analysis and visualization pipelines. Use pre-configured analysis pipelines and standard assays or build customized workflows to achieve specific goals.


Boost your productivity for these and more analyses:


  1. Advanced 3D analysis
  2. High content analysis
  3. Tracking and lineage
  4. Neuron tracing


Long-term imaging of sensitive processes: Zebrafish ear undergoing developmental morphogenesis


Eligible for a Lightfield 4D upgrade on the camera port:


  1. LSM 900 on Axio Observer 7
  2. LSM 910 on Axio Observer 7
  3. LSM 980 on Axio Observer 7
  4. LSM 990 on Axio Observer 7


Contact your local ZEISS representative to check additional requirements and for more information.



Zebrafish embryo, timelapse movie of developing otic vesicle, 2 – 3 days post fertilization, otic epithelial labelled with GFP-CAAX, nuclei with RFP. Every 2 minutes, volumes of 4 different zebrafish embryo ears were imaged over the course of 16 hours. Courtesy of Tanya Whitfield, Sarah Baxendale, School of Biosciences, University of Sheffield, UK. Data acquired at the Wolfson Light Microscopy Facility, University of Sheffield.


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Carl Zeiss Microscopy GmbH

07745 Jena, Germany

[email protected]

www.zeiss.com/lightfield-4d


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Not for therapeutic use, treatment or medical diagnostic evidence. Not all products are available in every country. Contact your local ZEISS representative for more information.


EN_41_012_363 | Version 1.0 | CZ 04-2026 | Design, scope of delivery, and technical progress subject to change without notice. | © Carl Zeiss Microscopy GmbH

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Lightfield 4D Upgrade for LSM 9 Series Systems

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