Intestine-on-a-chip infected with Vibrio Cholerae. blue = DAPI, green = bacteria, red (Cy3) = E-cadherin, far red = ZO-1 (tight junctions).

40x/1.1 objective used for a volume of 361 x 361 x 109 µm using Lightfield 4D to capture 4 channels.

LF4D helped the user quickly get a comprehensive overview of the entire sample.

Sample courtesy of Adrian Feile and Manuel Allwang, University Hospital Jena, Germany