Explore the latest advancements in multi-color live cell imaging. This approach enables simultaneous analysis of multiple cellular markers, enhancing research capabilities in dynamic cellular environments.
Revolutionizing Live Cell Analysis with 5-Color Fingerprinting
Key Takeaways
- Research focus: Multi-color live cell imaging for cellular marker analysis.
- Model system: Live cells analyzed with 5-color techniques.
- Research goal: Enhance cellular marker identification and analysis.
- Presented by: ZEISS R&D team.
- Content type: Product Highlight on advanced imaging techniques.
LLC-PK1 cells (pig epithelial kidney cell line) expressing Tubulin-mEmerald (Tubulin, cyan) and H2B-mCherry (Histone-bound DNA, white), additionally labeled with Mitotracker Orange (Mitochondria, yellow), SiR-Actin (Actin, pink) and DOPE-ATTO 740 (vesicles, orange).
Top: Excitation spectra of the 5 labels
Left: Live cell imaging (timelapse) with 5 colors imaged simultaneously and unmixed in real-time using Online Fingerprinting, processed with LSM Plus
Right: Single unmixed labels
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LLCPk1 - Organelles | Spectral LSM Plus
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