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Explore dynamic imaging of cellular elements using Lattice SIM, capturing mitochondrial and calreticulin interactions. This approach enhances our understanding of cellular function and structure with minimal phototoxicity.

Real-Time Imaging of Mitochondria & Calreticulin with diSIM

Key Takeaways

  • Research focus: Live-cell imaging of mitochondria and calreticulin using diSIM.
  • Model system: Cellular imaging with subcellular resolution.
  • Research goal: Observe rapid cellular interactions and processes.
  • Content type: High-resolution imaging analysis.
  • Image: Mitochondria and calreticulin dynamics captured in real-time.
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2 min read

Cos-7 cell expressing the endoplasmic reticulum marker Calreticulin-tdTomato (magenta) and mitochondrial marker Tomm20-mEmerald (green) was simultaneously imaged for two colors. The movie shows high dynamic interactions of the ER and mitochondria. Objective: Plan-Apochromat 63× / 1.4 Oil

https://zeiss.widen.net/s/t8zxmxswkf/sb1micro-signm_mm_calreticulintomm20-simultan-15ms-120timepoints-_disim-roi3_b0v0t43z0c0-2x0-1505y0-1005
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Cos7 - Mito + ER | Lattice SIM

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