This video explores the interactions between fungal infections and epithelial tissues using advanced imaging techniques. Insights into the fungal invasion process help understand host-pathogen dynamics crucial for therapeutic development.
Dynamic Imaging of Fungal Epithelium Interaction
Key Takeaways
- Research focus: Interaction of fungal infections with epithelial tissues.
- Technique: Advanced imaging highlights infection dynamics.
- Research goal: Understand host-pathogen interactions for therapy.
- Presented by: ZEISS collaborative research team.
- Content type: Scientific Video on pathological imaging.
Epithelial cell layer infected with fungal isolates. Labelled with phalloidin (AF647), anti-fungal (AF488) and Hoechst.
Acquisition Parameters:
LD 40x/1.1 objective used for a volume of 361 x 361 x 109 µm per position in a 16 position array acquired in a total of 19.5 seconds, including 3 channels, 50 ms exposure per channel, using Lightfield 4D
In large tissues such as this one, it can be a challenge to find the areas of interest that are worth pursuing further without time-consuming and laborious z-stack acquisition when using traditional imaging methods. Here, researchers are interested specifically in the sites where the fungal infection penetrates the epithelium cell layer, but finding these rare events can be a challenge.
High-speed, 3D imaging with Lightfield 4D enables ultra-fast 3D scanning of large tissue areas in order to quickly find the 3D structures of interest. In this case, locations of penetration of the fungus into the epithelial layer are quickly found in a matter of seconds, enabling high-speed targeted follow up with high resolution imaging modalities such as Airyscan.
Dr Emily Jones, Quadram Institute Bioscience.
