Explore the dynamics of GFP fusion protein expression in living cells using the ZEISS Celldiscoverer 7. This timelapse captures cellular activities in real-time, offering valuable insights into protein dynamics and cellular processes.
Dynamic Insight into GFP-Expressing Cells with Celldiscoverer 7
Key Takeaways
- Research focus: GFP fusion protein dynamics in live cells with Celldiscoverer 7.
- Model system: Cells expressing GFP fusion proteins.
- Research goal: Understand cellular processes through protein expression visualization.
- Presenter: ZEISS expert in imaging solutions.
- Content type: Product Overview demonstrating dynamic cell imaging.
24hr timelapse of a co-culture of 2 populations of HEK293 cells expressing split GFP. Cells were transfected 24hrs after seeding with plasmids expressing p14, a reovirus fusogen, and a transfection marker (H2B-mRFP). The appearance of green fluorescence signals the fusion of beighboring cells and reconstitution of the split-GFP molecules in one cytoplasm. (CD7 - 20x/0,95 x0,5, Orca Flash_4.0)
Movie courtesy of Victoria Deneke - Research Institute of Molecular Pathology - IMP, Vienna, Austria.
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HEK293 - Cytosolic | Widefield
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