Utilizing the Axioscan 7, this study focuses on fluorescence imaging of tumor invasion fronts spatial applications. The system enables detailed visualization and analysis of tumor boundaries, providing insights into pathology and cancer progression.
Visualizing Tumor Invasion with Axioscan 7
Key Takeaways
- Research focus: Fluorescence imaging of tumor invasion fronts.
- Model system: Human tissue samples for cancer research.
- Research goal: Analyze tumor boundaries to understand progression.
- Presented by: ZEISS research team.
- Content type: Clinical imaging application overview.
Oral carcinoma tissue with 6 cell specific antigen labelings. Sample: N. Gaßler / A. Berndt / D. Samsel, University Hospital Jena, Section Pathology
Opal dyes. Opal 780: Vimentin = mesenchymal cell marker (purple); Opal 690: Cytokeratin = epithelial cell marker (red); Opal 620: CD20 = B lymphocyte marker (pink); Opal 570: CD31 = endothelial cell marker (yellow); Opal 520: CD4/CD8: T lymphocyte marker (light blue); Opal 480: alpha smooth muscle actin = smooth muscle cells and myofibroblasts (green); DAPI: nuclei (dark blue)
Red (690): Cytokeratin, all epithelial cells, invasively growing tumor cells/tumor cell complexes
Green (480): Activated tumor stroma/tumor-associated fibroblasts
Left side:
Magenta (620): B lymphocytes
Cyan (520): T lymphocytes
Purple (780): Vimentin: mesenchymal cells
