This video showcases time-lapse imaging of Lifeact-labeled U2OS cells using structured illumination microscopy (SIM). The focus is on capturing dynamic cellular processes in three dimensions, and higher resolution for better analysis.
Dynamic 3D Imaging of Actin Cytoskeleton with SIM
Key Takeaways
- Research focus: Time-lapse 3D imaging with SIM.
- Model system: Lifeact-labeled U2OS cells.
- Research goal: Analyze dynamic cellular processes.
- Presented by: ZEISS imaging team.
- Content type: Visual demonstration of SIM capabilities.
Actin dynamics in a U2OS cell expressing LifeAct-GFP were imaged with the SIM Apotome 3D Leap mode and reduced phases. The image shows a maximum intensity projection of the volume stack. Objective: Plan-Apochromat 40× / 1.4 Oil
Play
U2Os - Actin | SIM Apotome
Related To Cell Biology
Content For Core Facility Managers
Content For Cell Culture
Similar to U2Os - Actin | SIM Apotome
Newest Content
Powered by Navless.ai