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This content highlights the use of SIM technology for imaging fixed actin-tubulin structures, emphasizing high-resolution analysis through color-coded projections. It provides detailed information for researchers focusing on cellular architecture.

Actin-Tubulin Imaging with SIM: High-Resolution Insights

Key Takeaways

  • Research focus: Imaging fixed actin-tubulin with SIM for detailed cellular structure.
  • Model system: Fixed cellular samples with actin and tubulin markers.
  • Research goal: Analyze cellular architecture using color-coded projections.
  • Presented by: ZEISS experts with advanced imaging solutions.
  • Content type: Scientific overview of imaging techniques.
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2 min read

The Lattice SIM² images of Cos-7 cells labeled via immunofluorescence with anti-alpha-Tubulin Alexa 488 are shown as color-coded ­projection. Data were acquired with Plan-Apochromat 100× / 1.57 oil (left) and Plan-Apochromat 63× / 1.4 oil (right) objectives.

The images demonstrate the excellent sectioning capabilities of SIM² image reconstruction algorithm.

https://zeiss.widen.net/s/nxzdvcsscv/sb10micro-signm_fixed-actin-tubulin-63x-13phases-01_disim_std_ch1sh5_ch2sh3_w0_02_proc3-color-coded-projection-11
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Cos7 - Actin + Tubulin | Lattice SIM

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