This content highlights the use of SIM technology for imaging fixed actin-tubulin structures, emphasizing high-resolution analysis through color-coded projections. It provides detailed information for researchers focusing on cellular architecture.
The Lattice SIM² images of Cos-7 cells labeled via immunofluorescence with anti-alpha-Tubulin Alexa 488 are shown as color-coded projection. Data were acquired with Plan-Apochromat 100× / 1.57 oil (left) and Plan-Apochromat 63× / 1.4 oil (right) objectives.
The images demonstrate the excellent sectioning capabilities of SIM² image reconstruction algorithm.



